Optical coherence tomography using an optical probe with a diameter of 0.9 mm, combined with the 14-18 gauge needle, is used for clinical needle puncture. Using the real-time image obtained from the tip of the needle in the tissue, the needle position can be identified. Combined with artificial intelligence can achieve objective, accurate and automatic identification of the tissue, which has been successfully verified in anesthesia and laparoscopic surgery.

Our solution is able to noninvasively reconstruct 3D skin microstructuremicroangiography with micrometer-scale resolutions for diagnoses of skin disorders. Moreover, various skin parameters can be quantitatively evaluated from 3D images according to the proposed algorithms, including the thickness of stratum corneum, the thickness of epidermis, water concentration, collagen concentration,skin roughness. The developed technique can be applied to different clinical environments including hospitalscosmetic clinics for diagnosesanalyses of skin.