Induced pluripotent stem cells are a promising new technology in translation medicine,AI technology is an automaticpowerful platform that can help identify cell images with unique characteristics, predictprovide information about cell types, differentiation stages, cell quality,normal Or disease-specific feature information. The combination of this induced pluripotent stem cell differentiation platformAI-based artificial intelligence image analysis technology will have great benefits for future cell product applications.

Epidermolysis bullosa (EB) is an inherited blistering skin diseaseis also one of the most "urgent, severe, difficultrare" diseases. Since there are at least 21 disease-causing genes involved in EB with markedly variable manifestations, it is particularly difficult for accurate diagnosis. In recent years, next generation sequencing (NGS) has been widely applied in the genetic diagnosis of rare hereditary disorders. The EB team in NCKU established the protocol of EB diagnosis by combining NGSvarious tools of pathologymolecular studies. Till now, we have completed the genetic diagnosis for more than 50 Taiwanese EB patients.