The present sperm sorting chip utilities the upstream swim characteristic of sperms in appropriate fluid velocity to achieve up to 80 high motility sperm yield for infertile males in just 40 minutes

CRISPR/Cas9 is the powerfulpopular genome editing technology which can recognize target gene by a single guide RNA with the advantages of precision, high efficiency, easy manipulation,low cost to be applied to promote the precision breeding of aquatic organisms. The potentials of genome editing in the precision breeding of ornamental fish are mainly focused on infertility control, alternation of colorshape, growth promotion, sex control, stress tolerance, virus resistance, etc.

We developed a new type of microfluidic chip with utilizing the characteristic of sperm anadromous instinct for high-throughput sperm separation. The process can be finished in 10 min, which is 10 times faster than the conventional design. Experiment reveals that the qualified sperm motility was raised up from 30 to 99linearity (LIN) up from 0.2 to 0.85 via sorting by our microfluidic chip.

Infertility is a serious globe issue, especially in the developed countries, over 20%infertile male has sperm problems, that include instance immotile, abnormal morphology and low sperm quantity. Therefore, how to isolate high quality sperm for clinical use is extremely required. Up to date, activity sperm sorting is usually using Swiming-up and Density gradient centrifugation approaches. Unfortunately, both methods are detrimental to the sperm viability and elicits production of reactive oxygen species. Separation sperm by microfluidics has been put emphasis on and studied; however, it faced some challenge such as low throughput and an unacceptable recover rate.