Photonic_Workshop@CCMS.NTU has successfully developed a motorized micro-mapping system to explore and navigate samples. This system is able to acquire high resolution images to produce a sample map automatically. This map is also synchronized with the coordinates of motorized stages. This achievement allows users to explore and position their hotspots from the map, and precisely deploy the hotspot to the central field of view by motorized stages. In addition, this mapping system can combine with a laser scanning confocal spectral microscope (LSCSM) to acquire sample spectral mapping. Our result has shown the capability to improve the efficiency of hotspot positioning and data acquisition process.

EPICAn is a novel technique that uses liquid biopsy for epigenetic analysis. Using methylation-specific PCR method to analyze specific gene methylation of cell-free DNA in plasma for early detection of breast cancer. The advantages of this technology are 1. High accuracy 2. Low false positive rate 3. Low-invasive 4. less limitation 5. Available for routine continuous detection 6. Monitoring cancer changes 7. Aided diagnosis 8. Automated process 9. Easy-to-operate 10. Rapid analysis. It is expected that EPICAn technique can increase the accuracy of early detection of breast canceraid clinicians diagnosis.

Optical coherence tomography using an optical probe with a diameter of 0.9 mm, combined with the 14-18 gauge needle, is used for clinical needle puncture. Using the real-time image obtained from the tip of the needle in the tissue, the needle position can be identified. Combined with artificial intelligence can achieve objective, accurate and automatic identification of the tissue, which has been successfully verified in anesthesia and laparoscopic surgery.

NCREE has originally developed 5D digital space—on the basis of 3D city modelsconnections of different kinds of sensors around the world— is an online to offline virtual space with a combination of rising 5G technology advantages. Collectingreorganizing various 3D cartographic data with Building Information Modeling (BIM), satellite imagery, UAV 3D modeling, LiDar point cloud data, etc., can increase the diversity of the buildinglandscape. 5D+5G smart city platform would accelerate the 5D smart city to become the digital twin of a real city.

This technique uses multimodal nonlinear optical microscopy with the image contrast of second harmonic generation (SHG)coherent anti-Stokes Raman scattering (CARS). By analyzing the nonlinear signals on images, it can be used to characterizequantify the structural variation in the collagen scaffolds processed with different crosslinking methods. The correlation between signalsmechanical properties can then be derived. Additionally, comparative analysis of these two signals can be used to monitor the structural (triple-helix) change of collagen molecules during the crosslinking processes.