PRRSV recombinant antigen A1 inhibits the expression of CD163 on the cell surface by inducing the polarization of M1 macrophage subset, which can further effectively reduce virus invasionreduce infection. A1 can regulate the endogenous genes of alveolar macrophages to activate Th1-type immune responseenhance cellular immunity. Therefore, this technical invention will be beneficial to screen the new generation of PSSRV candidate structural vaccines.

PCR, a gold-standard diagnostic method, were labor-intensive, time-consuming,costly, which restricted its application to widespread screening. Herein, this study purposes a one-potnon-washing method to rapidly detect virus by dual-SERS mechanism. COVID Antigens were captured by SERS nanoparticlesnovel SERS substrate simultaneously to achieve 6 order enhancements within 20 minutes. The dual-SERS sensors have reached a detection limit of 1 ng/ml in clinical samples for recognizing nucleocapsid & Spike proteins of COVID-19, which is comparable with PCR results.

Provided is an influenza mucosal vaccine compositionpreparationapplication thereof. This composition contains an antigen fusion protein which includes an influenza virus antigena Type IIb heat-labile enterotoxin A subunit. Immunization with the antigen fusion protein induces cellularhumoral immune responses, including systemicmucosal immune responses, against a specific in

Due to the recent outbreak of COVID-19ongoing mutants, we developed a coronavirus variant protein microarray that includes all the current spike mutationsthe wild-type viral antigensapplied in detecting immune responsesdrug specificities. We filed the patientspublished our findings in the Analytical Chemistry. Besides massive production of the coronavirus protein microarray, we also collaborated with a scanner company, developed artificial intelligence software,collaborated with suppliers. We are welcome any sort of collaboration.

The invention provides a glycan-masking and/or glycan-unmasking hemagglutinin, wherein the glycosylation site(s) is added on globular headremoved on stem. The glycan-masking and/or glycan-unmasking hemagglutinin may induce neutralizing antibody against influenza virus,cross-reactive protection against different virus. The invention also provides a method for manufacturing these antigens.

In this project, we have reorganized several highly immunogenic sitesstructurally essential regions to maintain it in a pre-fusion stateuse it as main antigen in our RSV vaccine candidate (RSV-F005). To induce a mucosal immune response, intranasal administration route was chosen. New mucosal adjuvant (H-muad) has been tested in threee animal models.

A high-sensitivity quantitative ELISA detection system for the large HBV surface (LHBS) protein was developed. It includes monoclonal antibodies for LHBSchemiluminescent sandwich ELISA method. Clinical tests of the ELISA prodcuts have been performed in CHB cases. The LHBS detection methodsproducts have been filed for US provisional patentwill be submitted for the IVD approval.

We developed a biomimetic triple-antibody-immobilized magnetic fucoidan nanomedicine as a multifunctional artificial antigen presenting cell, which possessed the ability to not only inhibit immune checkpoint but activate tumor infiltrated T cells. of Bridging sites with tunable density on the nanoplatform was designed, allowing the antibodies to be well-distributed on the surface for mimicking immune cells. In contrast to the complex cell expansion process using microbeads in adaptive cell therapy, the nanoplatform can be i.v. administrated to cut the course of therapy from several weeks to days. With the development of the platform technology, an artificial immune system family can be built to pave the way for personalized immunotherapy.

Effectively inducing cytotoxic T cell expansion has been a critical challenge in vaccine development. To address this challenge, an entirely biodegradable polymeric nanoshell was invented to couple antigensadjuvants for safepotent immune potentiation. The invention has been adopted for precision anticancer vaccine, broadly reactive influenza vaccine,an effective vaccine MERS-CoV.

"The IHC staining intensity grading algorithm combines a series of complementary processes of deep learningimage processing in order to overcome the difficulties in cell boundary segmentationgrade definition,provides a cell-based IHC staining intensity gradingquantification capability. The proposed method serves as a useful assistive tool for physicians in performing accurate staining quantification in tissue microscopic images. In addition, it analyzes panoramic pathological images in order to determine the factors most strongly associated with likely disease recurrence."