We designed a cascade spiral microfluidic device (i-Finity®)continuously isolated the unlabeled cancer cells from blood samples without lysing processes. The results showed that the i-Finity® device excluded a high percentage of RBCs in the first stagerecollected the cancer cells in the second stage. The i-Finity® device can efficiently enrich the cancer cells more than 1000 times in 10 minutes, which substantially curtailed sample preparation timeimproved the accuracy of diagnosis.

To optimize particle focusing conditions in the cascaded platform, two-stage spiral microchannels are designed with different heights. This allows the i-Finity® system to operate at high efficiency, removing 99.99 of red blood cells without antibody labeling. In addition, it can also collect over 80 of cancer cells effectively under a flow rate over 1 mL/min. In conclusion, this device provides a fast, high-throughput, low-cost,preconcentration platform for cancer cell detection technologies.

We designed a cascade spiral microfluidic device (i-Finity®)continuously isolated the unlabeled cancer cells from blood samples without lysing processes. The results showed that the i-Finity® device excluded a high percentage of RBCs in the first stagerecollected the cancer cells in the second stage. The i-Finity® device can efficiently enrich the cancer cells more than 1000 times in 10 minutes, which substantially curtailed sample preparation timeimproved the accuracy of diagnosis.

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