Alcalase comes from the endoprotease of Bacillus licheniformis, which has low specificity, mainly the C-terminal hydrophobic amino acid. Hydrolysis of protein by enzymes will release peptide fragments with physiological activity, which can be used as regulatory functional foods with physiologically active functions in intestinal digestion, and the use of molecular weight cut-off (MWCO) and separation and purification analysis. That can further enhance its active concentration and its functions, such as lowering blood pressure, anti-Nonalcoholic fatty liver, lowering lipids and lowering blood sugar. The potato protein hydrolysate (APPH) was analyzed by liquid chromatography tandem mass spectrometry (LC / MS / MS); the signal spectrum of the mass spectrum was compared with the amino acid sequence of the main storage protein Patatin in the potato as a benchmark and then the identified novel potato biologically active peptides are synthesized and purified to function verification studies.

Natural bioactive peptides are considered to be specific protein fragments. They can be released by enzyme hydrolysis and play various physiological functions; Bioactive peptides are released after passing through the gastrointestinal tract and has a variety of special physiological regulating effects. The researches on  bioactive peptides or protein hydrolysates with different physiological activities have increased rapidly in the past few years. In this study, the efficacy of potato bioactive peptides has the same effect as treatment drugs ( hyperlipidemic, hypoglycemic and hypertension drugs are in the positive control group), and have a three-in-one effect, and these peptides will not accumulate residues and have no side effects in the body and the potential to be  a functional food.

The potato protein hydrolysate was analyzed by liquid chromatography tandem mass spectrometry (LC / MS / MS); the signal spectrum of the mass spectrum was compared with the amino acid sequence of the main storage protein Patatin in the potato as a benchmark. and then the identified novel potato bioactive peptides are synthesized and purified to function verification studies.