The platelet-rich plasma (PRP) clinical screening platform provides scientific data to physicians to evaluate the best PRP prolotherapy. Tenocytes, periosteal cells, and PRP can be co-cultured under the common medium to simulate the healing interface environment after the PRP injection. During the culture course, cell proliferation is quantified by the impedance technique. Then, the artificial intelligence expert system optimizes the PRP prolotherapy.

The development is a technology to optimize PRP prolotherapy for individual patient. By collecting the tissue sample and blood, primary tenocytes, periosteal cells, and PRP can be co-cultured to simulate the healing interface environment after the PRP injection. Cell proliferation is quantified by the impedance technique. Then, the artificial intelligence expert system determines the efficacy of PRP prolotherapy.

Although PRP prolotherapy has been academically recognized, clinical applications remain controversial. Due to the differences of individual patients, the efficacy of the PRP prolotherapy cannot be confirmed. In the industrial applications of arthroscopic surgery, sports medicine, and medical cosmetology, this technology provides a reliable and objective automated, high-throughput, sample-less optimization screening platform for PRP prolotherapy.