The coloration rateEa of the reaction between guaiacollaccase TTI, prepared by immobilizing laccase with electrospun fiber with high specific surface area, can be manipulated by adjusting the amount of laccase, pHNaN3. The laccase TTI exhibits high accuracy, stability, temperature reversibility, low cost,can be applied in intelligent food packaging to monitor food quality.

The laccase immobilized with electrospun nanofibers can be a solution to overcome the defects, high costinstability, of enzymatic TTI. The high specific surface area contributed to reduce the enzyme dose the immobilization contributed to the enzyme stabilitytemperature reversibility the kinetic model contributed to the accuracy of the of food quality monitoring deterioration.

The laccase TTI exhibits high accuracy, stability, temperature reversibility, low cost,can be applied in intelligent food packaging to monitor food quality by accumulating information on timetemperature to the managers of food manufacturer, logistics, vendors,consumers. Its cost-effective, friendly to observer design can monitor quality information on food to reduce risk in the cold