This technique used algae-a natural raw ingredient as novel functional aquafeed additives, as replacement for fish meal in nutrition sources, a fish oil replacement in the feed, an algae strain contains unsaturated fatty acid can change level of serotoninblood cortisol in fish brain, decrease their cannibalism rate. This technique can reduce feed production costincrease harvesting rate.

Two parts in this integrated creative invention: deep processing for frozen aquatic meal productshigh-valued utilization for byproducts. The deep processing technology for fish meal is the commodities without thawingwashing process while cooking. The technology also can remove fishy smells. Byproducts are full of rich nutrition but usually were used as animal feeding food, sold by low prices,evenly discarded. This technology is developed to extract nutrition factors in fish bone/scales by using an autoclave system with a series of heatingpressure procedures.

CRISPR/Cas9 is the powerfulpopular genome editing technology which can recognize target gene by a single guide RNA with the advantages of precision, high efficiency, easy manipulation,low cost to be applied to promote the precision breeding of aquatic organisms. The potentials of genome editing in the precision breeding of ornamental fish are mainly focused on infertility control, alternation of colorshape, growth promotion, sex control, stress tolerance, virus resistance, etc.

This invention targets at value-added increase for fish bone/scale by-products through a series of manufacturing units of steaming, pressure/ temperature,drying. The core design of pressure/temperature in terms of powdered machining equipment consists of a high pressure chamber, heating components, superheated steam,operations experiments as well.

A method of enhancing a mammalian immune response to a virus is disclosed. The method comprises administering a composition comprising an effective amount of epinecidin (Epi)-1the virus to a mammal, wherein the virus has envelope proteinis infectious to the mammal. In this technique, Epi-1Japanese encephalitis virus (JEV) were mixedinjected into mice. The mice were infected with JEV 14 days later,all mice survived. This technology has also been verified in the preparation of vaccines for foot-and-mouth disease virusnervous necrosis virus.

The electrochemical sensor can detect bioaminesall amino acids without chemically labeling proceduresenzymatic catalysisbe capable of performing the measurement in pH-varied solutions . The electrodes can be modified with a specific membrane for the selective detection of histamineintegrated with a separation device to perform high selective detection of multi-analytes.