We developed a recombinant adenovirus Ad-HFMD vaccine which can express virus-like particles (VLPs) of EV71 capsid proteins. Mouse studies showed that Ad-HFMD can induce the protective antibodycellular immunity against EV71CVA16 in hSCARB2 transgenic (hSCARB2-Tg) mouse challenge model.

CRISPR/Cas9 is the powerfulpopular genome editing technology which can recognize target gene by a single guide RNA with the advantages of precision, high efficiency, easy manipulation,low cost to be applied to promote the precision breeding of aquatic organisms. The potentials of genome editing in the precision breeding of ornamental fish are mainly focused on infertility control, alternation of colorshape, growth promotion, sex control, stress tolerance, virus resistance, etc.

Our core generated several types of transgenic micea stable humanized ACE2 mice using a combi-CRISPR genetic engineering technique. We hope these mouse models can help to dissect COVID-19 pathogenic mechanismthe interaction(s) between spike proteinACE2 K353.