A stem cell continuous harvest method was proposed. In this method, stem cells are partially detached. The remaining cells are continuously cultured by expansion in fresh culture medium.

Induced pluripotent stem cells are a promising new technology in translation medicine,AI technology is an automaticpowerful platform that can help identify cell images with unique characteristics, predictprovide information about cell types, differentiation stages, cell quality,normal Or disease-specific feature information. The combination of this induced pluripotent stem cell differentiation platformAI-based artificial intelligence image analysis technology will have great benefits for future cell product applications.

The invention integrates a variety of technologies including stem cell proliferation, directed inductioncryopreservation in vitro. This integrated system was developed under chemical defined condition,can expand various type of stem cells over one hundred foldcan furtherly induce to specific mature cells up to ten millions. In addition, the amount of cryoprotectant can be significantly reduced to 2 in the cryopreservation system with excellent cell survival rate. This integrated system can provide a promisingvarious stem cell sources for cell therapyimmunotherapy.

Gene delivery technology is widely used in cell therapy, nucleic acid-based vaccines, protein production, gene therapy, etc. We have developed a non-viral gene delivery technology-MPGene, which has excellent gene delivery efficiency (50-80) for human mesenchymal stem cells,also has the characteristics of low production costhigh safety. In the future, MPGene has the potential to be used in important biomedical fields such as gene delivery reagents, major trauma cell therapy, malignant tumor treatmentcartilage cell therapy.