PCR, a gold-standard diagnostic method, were labor-intensive, time-consuming,costly, which restricted its application to widespread screening. Herein, this study purposes a one-potnon-washing method to rapidly detect virus by dual-SERS mechanism. COVID Antigens were captured by SERS nanoparticlesnovel SERS substrate simultaneously to achieve 6 order enhancements within 20 minutes. The dual-SERS sensors have reached a detection limit of 1 ng/ml in clinical samples for recognizing nucleocapsid & Spike proteins of COVID-19, which is comparable with PCR results.

Surface-enhanced Raman spectroscopy (SERS) is a useful analytical technique for detecting extremely small amounts of molecules. Herein, we designed a paper-based quasi-three-dimensional SERS substrate (P-SERS) that can provide potential to improve Raman analyses for food safety, pesticide poisoning, precision medicine, drug abuse and DNA/RNA testing. The sensitive, low-cost, flexible and disposable SERS substrate could be easily fabricated by physical deposition of gold nanoparticles array onto a filter paper. In this case, we are able to create non-continuous Au islands on the fiber surfaces, where the gaps between AuNPs can dramatically generate the high electric field to enhance Raman signal of target molecules.

A portablemagnetic surface Raman-enhanced scattering (SERS) nanoparticle arrays platform would be developed for magnetic separationSERS detection of environmental, water,biomolecules samples. It is very simplerapid platform with portable Raman spectroscopy to detect samples (bacteria, virous, water toxicants) after magnetic separation processes, without labelling the fluorescence dye,the limit of detection concentration is lower than 10-8 M.