A method of enhancing a mammalian immune response to a virus is disclosed. The method comprises administering a composition comprising an effective amount of epinecidin (Epi)-1the virus to a mammal, wherein the virus has envelope proteinis infectious to the mammal. In this technique, Epi-1Japanese encephalitis virus (JEV) were mixedinjected into mice. The mice were infected with JEV 14 days later,all mice survived. This technology has also been verified in the preparation of vaccines for foot-and-mouth disease virusnervous necrosis virus.

Our PEDD has been used to detect the HNE level in both tearwound fluids of 9 patients,the limitations of detection were about 0.76 μg/mL0.63 μg/mL respectively. Our device requires only 15 minutes, 3 μL of clinical sample for each test,offers superior sensitivity compared to other current HNE detection methods. The HNE concentrations in the patient samples have shown that the elevated HNE levels might suggest an abnormal healing of either acutechronic wounds, consistent with previous studies that showed excess protease levels in all non-healing wounds.