Technical Name |
Using miRNAs to develop the molecular diagnosis method for Kawasaki disease |
Project Operator |
CHANG GUNG MEDICAL FOUNDATION |
Project Host |
郭和昌 |
Summary |
The diagnostic kit developed by our team uses real-time PCR to detect the expression levels of 10 kinds of miRNAs,then uploads the data to the computing website,the diagnosis result can be obtained within 1 minute. Machinesrelated equipment for instant polymerization reaction are very popular,general regional hospitals have this equipment. In addition, it takes less than 5 hours to obtain the final detection result from blood drawing,the cost of related consumablestrial meter is only less than NT$1,000. Therefore, the Kawasaki disease detection kit developed by our team fully conforms to the principles of accuracy, speedconvenience. And the first set in the world. |
Scientific Breakthrough |
The diagnostic kit developed by our team uses real-time PCR to detect the expression levels of 10 kinds of miRNAs,then uploads the data to the website for calculation. The diagnosis can be obtained within 1 minute. In addition, it takes less than 5 hours from blood drawing to obtaining the final diagnosis result,the cost of related consumablestrial meter is only less than NT$1,000. Therefore, the Kawasaki disease(KD) diagnostic kit developed by our team fully conforms to the principles of accuracy, speedconvenience. The successfully developed KD microRNA detection kit has been successfully transferred to sofiva genomics Companyis about to start clinical application. |
Industrial Applicability |
The diagnostic kit developed by our team uses real-time qPCR to detect the expression levels of 10 miRNAs,then uploads the data to the computing website, where the diagnosis can be obtained within 1 minute result. Machinesrelated equipment for instant polymerization reaction are very popular,general regional hospitals have this equipment. In addition, it takes less than 5 hours from blood drawing to obtaining the final diagnosis result,the cost of related consumablestrial meter is only less than NT$1,000. Therefore, the Kawasaki disease diagnostic kit developed by our team fully conforms to the principles of accuracy, speedconvenience. |
Keyword |
Kawasaki Disease miRNA Next Generation Sequencing real-time PCR |