Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infected human cell through ACE2. This study is developing a Pd nano-thin film electrode for electrochemical impedance spectroscopy. This invention can quantify neutralize antibody too. The detection platform can be used to screen 1 microliter anti-virus infection drug sample within 21 mins. Compare to SPR standard, our invention only needs 1/10000 concentration of ACE2reaction volume is ~1/30 than SPR. This invention can be adapted in detection of cancer markers,other emergency infection diseasestherapeutic drugs, too.

This invention is the novel biosensing platform is based on the interaction between spike proteinACE2 interaction. Therefore, the mutant spike proteins can be analyzed. Furthermore, the titer of neutralize antibody can be detected as well. Conventional ELISA cannot tell neutralize antibody from other binding antibodies. Furthermore, surface active biosensing electrode can direct immobilize ACE2 within 20 minthe volume of protein/drug samples are 1l each. Compare to SPR standard, our invention only needs 1/10000 concentration of ACE2reaction volume is ~1/30~1/100.

Surface active Pd nano-thin film based electrochemical platform is the key technique for SARS CoV-2 sensinganti-infection drugs screening. This technique which can conjugate biomolecules directly without linkers. The detection time is as good as SPR gold standard, but with ~1/10000 of concentration~1/30 of volume less than SPR. This invention can quantify neutralize antibody as well. Moreover, this technique can be adapted in detection of cancer markers,other emergency infection diseasestherapeutic drugs, too. Both health impactmarket value will be extremely high.

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