Technical Name |
Digital holographic microtomography system |
Project Operator |
National Taiwan Normal University |
Project Host |
鄭超仁 |
Summary |
Holographic tomography uses a laser beam scanning mechanism to record and reconstruct the three-dimensional refractive index distribution and tomographic structure of the biological samples used. This technology can achieve micron/nanometer-resolution in three-dimensions without the use of dye/markers, staining the sample and fluorescent labeling. For non-radiation damaging and non-invasive biomedical imaging in life science and other fields, this technology will be more beneficial for the researchers and scientific experts to study and analysis in more detail to understand the live cell behavior monitoring and diagnosis at its sub-cellular levels, biophysical parameter measurement, cell cycle studies etc.. |
Scientific Breakthrough |
1. Novel digital holographic micro-tomography with micron/nanometer level spatial resolution in three-dimensional label-free imaging and quantitative analysis. 2. Adaptive computer-generated holographic wavefront manipulation and aberration compensation methods to reduce the degradation effect of wavefront errors to achieve high-resolution, high-contrast holographic microtomography. 3. Live cell measurement at its native culture environment provides non-invasive, non-radiative and novel optical super-resolution imaging and diagnosis method without using any additional dyes and fluorescent markers. |
Industrial Applicability |
1. Three-dimensional quantitative imaging and analysis, phase and refractive index distribution of the samples used with micron/nanometer lateral and axial resolution. 2. High-speed scanning optics and image processing algorithms to generate high-quality and quantitative 3D tomographic images. The system has high stability without the need for vibrational-proof optical tables. 3. The use of visible light band low-power laser allows non-contact, non-fluorescent marking and non-radiation damage measurement of live cell 3D images at its sub-cellular levels. 4. With customized module solution, it can be matched with other detection devices. |
Keyword |
Holography Digital holography Microscopy Tomography Three-dimensional imaging Living cell imaging Wavefront manipulation Aberration compensation Adaptive optics Spatial light modulator |