Animal vaccines are important for controlling infectious diseases. To reduce the economic losses caused by infectious diseases to the society, new biotechnologies must be applied to increase vaccine production efficiency. Therefore, there is an urgent need to develop a cost-effective cell culture process for the production of viral antigens.
This technology attempts ways to overcome the cell＇s interferon defense system for optimal virus replication. The CRISPR / Cas9 gene editing technology was used to delete the interferon related genes. 
This technology has applied on the BHK-21 cell line to delete the IFN receptor, and virus antigens with urgent needs were tested. In the production of IBDV and PEDV, the virus titers from the edited cells were 100 times higher than that from original cells. The technology could be a platform to assist development of IFN deleted cell lines that reducing animal vaccine production costs and enhancing the competitiveness of the industry.

The use of cells for virus production will be the main method for the production of viral antigens. Production cells are the key to this production system. This technology integrates many key technologies and analysis that can help to edit cells efficiently and easily. 
The overall technology includes three parts. First, the transcripts of cells after virus infection were analyzed for the growth and decline of various cytokines after virus infection, and then the potential cytokine genes that can boost the virus titer were identified by software. At step two, the CRISPR / Cas9 gene editing technology was applied to delete the related genes. Finally, this technology could amply the edited cells in the bioreactor for producing virus antigens.

This technology attempted to explore ways to overcome the cell＇s interferon defense system for optimal virus replication and bioreactor amplification, and to improve viral production process.
This technology has applied on the BHK-21 cell line to delete the TFN receptor, and virus antigens with urgent needs in the animal vaccine market were tested. In the production of IBDV and PEDV, the virus titers from the edited cell line were 100 times higher than that of original cell. The use of cells for virus production will be the main method for the production of viral antigens. This technology integrates many key technologies and analysis ways that can edit cells for an easier and more economical virus production system.