In this study, we wish to enhance gene engineering efficiency in E. colicyanobacteria by using CRISPRCRISPRi systemachieve the purpose of bio-chemical production. we final combined both CRISPRCRISPRi for metabolic engineering in E. colicyanobacteria, which improved the 1,4-BDO productionsuccinate.

"(1) CRISPR/Cas9 can trigger foreign gene integrates into the genome of E.colicyanobacteria.
(2) By using CRISPR system in E.coli, we enhance the titer of 1,4-BDO from 0.9 g/L to 1.8 g/L(about 100 increasing)final succinate production reached 15 g/L in 48hr.
(3) In cyanobacteria, CRISPR system can enhance the succinate production about 12.5 fold (≈0.58-0.63 mg/L)"

Recently, the key technologies for biochemical production (such as genetic engineering, metabolic engineering, synthetic biology,metabolomics) is weaker than the others.  Therefore, this research can also be used as a technical basisfurther apply to develop more effective biochemical production technologyenhance international competitiveness.